Tinna Berg Larsen
Skibby
Summary
Broad knowledge of cells and assays (biomarkers), specialist i development of automated assays, curious, helpful, team player
Overview
30
30
years of professional experience
Work History
Lead Laboratory Technician
Novo Nordisk
12.2023 - Current
- Development of genome edited cell clones on Biomek i7
- Genome integrity testing using ddPCR
- Copy number variation testing using ddPCR
- Making Out of frame indels using Cas9 endonuclease
Senior Laboratory Technician and Assay Development and Automation Specialist
Blue Cell Therapeutics
10.2020 - 11.2023
- Developed cells for use as regenerative medicine in damaged tissue
- Characterization on flow cytometry, Miltenyi, MacsQuant 10
- Optimization and cultivation of stem cells in 2D culture
- Sparring partner for when colleagues need to develop new assays/techniques/processes
Product Specialist in BMGLabtech Plate Readers and Field Application Specialist in Beckmann Coulter Biomek Liquid Handling
Ramcon
11.2018 - 10.2020
- Sale of plate readers from BMG Labtech
- Method writing and troubleshooting on Biomek robots from Beckman Coulter for our customers in various applications
Special Laboratory Technician
Novo Nordisk A/S
02.2000 - 10.2018
- Super user on Beckman Coulter robot Biomek Nxp for pipetting work in 96 and 384 well plates
- Receptor binding on SPA beads and whole cells
- Insulin and GLP1 secretion analysis on various cell lines
- Measurement of cAMP activation in various cells
- Functional assays on G-protein coupled receptors and ion channels
- Purification of proteins from cells and characterization using western blot
- Cell cultivation and transient and stable transfection of cells
- Various cell-based assays in 96- and 384-well plate format
- General molecular biological methods for the production and purification of DNA and RNA
- RT q-PCR
- Assay and method development
- Ca-imaging on primary neurons from the hypothalamus and hepatocytes
- Electrophysiological measurements on oocytes from frogs, on different cell lines and primary cells
- Operation and perfusion of experimental animals
- Supervisor for laboratory students, 2 years and for a refugee in 2 and ½ month in the Novo Nordisk intern program for refugees
- Broad knowledge in Excel, Word and Graph Pad Prism (statistical software)
- Co-author on publications
- Per Arkhammer, Philip Wahl, Tinna Fremming, Berit Gerlach, John B. Hansen Establishment and Application of in Vitro Membrane Potential Assays in Cell Lines with Endogenous or Recombinant Expression of ATP-Sensitive Potassium Channels (Kir6.2/SUR1) Using a Fluorescent Probe Kit, Journal of Biomolecular Screening
- Flemming E. Nielsen, Søren Ebdrup, Anette Frost Jensen, Lars Ynddal, Thora B. Bodvarsdottir, Carsten Stidsen, Anne Worsaae, Harrie C. M. Boonen, Per O. G. Arkhammer, Tinna Fremming, Philip Wahl, Hanne T. Kornø, and J. Bondo Hansen, New 3-Alkylamino-4H-thieno-1,2,4-thiadiazine 1,1-Dioxide Derivatives Activate ATP-sensitive Potassium Channels of Pancreatic Beta Cells, Journal of Medicinal Chemistry, Vol. 49 (14), 4127-4139, 2006
- M. Dabrowski, T. Larsen, F. M. Ashcroft, J. Bondo Hansen and P. Wahl: Potent and Selective Activation on the Pancreatic Beta-cell Type KATP Channel by Two Novel Diazoxide Analogues. Diabetologia, Vol. 46, 1375-1382, 2003
Laboratory Technician
Zealand Pharmaceuticals
05.1999 - 12.1999
- Solid phase synthesis of peptides
- Purification and characterization on preparative and analytical HPLC
- Stability test of peptides against enzymes on HPLC
- Development Agency (KVL) (6 months limited time)
Laboratory Technician
Novo Nordisk A/S
04.1999 - 04.1999
- Testing of insulin pens
- Reporting in collaboration with the consulting firm Carl Bro A/S
Research Laboratory Technician
PNA Diagnostics A/S
01.1995 - 04.1999
- Solid phase synthesis of PNA and peptides
- Analytical and preparative HPLC
- Mass spectrometry on MALDI TOF
- Radioactive kination of DNA and PNA (Peptide Nucleic Acid)
- Analyzes of PNA/DNA complexes
- Purification of DNA from various agents using PNA
- Single base mutation detection using LNA (Locked Nucleic Acid) in ELISA
- ELISA detection of PCR material
- PCR optimization, general and quantitative
- Gel electrophoresis
- Anthraquinone photo immobilization in microtiter plates, including durability tests and quality control, work in clean rooms and benchtops
- Co-author on publications
- Koch T, Hansen HF, Andersen P, Larsen T, Batz HG, Ottesen K, Ørum H, Improvements in automated PNA synthesis using Boc/Z monomers, The journal of peptide research, 49(1):80-8, 1997
Education
laboratorie tekniker
Laborant Skolen I Hovedstadsområdet
Copenhagen12-1994
Skills
- Assay development
- Cell culture
- In Vitro Drug Discovery
- Automation
- PCR
- Out of Fram Indels using CRISP/Cas9
- NGS
- ddPCR
Training
Stays abroad via my employments
- 2019: Field Application Specialist annual meeting, Indianapolis, USA, 5 days
- 2016: Participation in the SLAS 2016 (conference for new technology), San Diego, 1 week
- 2014: User Meeting for Biomek in Stockholm, 2 days
- 2005: Stay as a trainee at the University of Medicine and Dentistry, New Jersey, training in dissecting and dissociating neurons from the hypothalamus in mice and rats, 2 weeks
- 2003: Stay in laboratory in London, England, training in patch clamp on brain incision, 1 week
Courses
- 2018-19: Our way of Selling, Grape/Ramcon, Denmark, 21 days in total
- 2019: Lifescience – Automation, Biomek I Series Customer Training for experienced, 2 days, Nyon, Switzerland
- 2019: Product & Application Training, BMG Labtech, Ortenburg, Germany, 5 days
- 2012+2013: Programming of Biomek and Liquid Handling and Biomek Continuum training, Beckman Coulter, Nyon Switzerland, 2x3 days
- 2011: Diabetes training for laboratory technicians, Novo Nordisk A/S, 3 days
- 2010: Biomek Nxp liquid handling, Beckman Coulter course in Nyon, Switzerland, 3 days
- 2009: Personal Insight, 3 days
- 2006: Graph Pad Prism software course, 1 day
- 2004: Laboratory trainee supervisor course, 2 days
- 2003: Course in laboratory animal science B
- 2003: Advanced techniques in molecular Pharmacology, 1 week
- 2002: Diabetes course A
- 2001: General biochemistry course, 15 x ½ day
- 2001: Ion Channel Workshop in Leeds, England, 1 week
- 2001: Receptors, 4 days
- 1998: ELISA techniques, 1-day
- 1998: Lotus Notes, 1-day
- 1997: Cloning, internal 1-day
- 1997: PCR techniques, internal 1-day
- 1996: MALDI TOF mass spectrometry, 1-day
- 1995: HPLC, 3-day
Hobbies and Interests
Dressage riding, house and garden, summer house in Nykøbing Sjælland, pets (1 dog and 1 cats)
Timeline
Lead Laboratory Technician
Novo Nordisk
12.2023 - Current
Senior Laboratory Technician and Assay Development and Automation Specialist
Blue Cell Therapeutics
10.2020 - 11.2023
Product Specialist in BMGLabtech Plate Readers and Field Application Specialist in Beckmann Coulter Biomek Liquid Handling
Ramcon
11.2018 - 10.2020
Special Laboratory Technician
Novo Nordisk A/S
02.2000 - 10.2018
Laboratory Technician
Zealand Pharmaceuticals
05.1999 - 12.1999
Laboratory Technician
Novo Nordisk A/S
04.1999 - 04.1999
Research Laboratory Technician
PNA Diagnostics A/S
01.1995 - 04.1999
laboratorie tekniker
Laborant Skolen I Hovedstadsområdet
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